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Lipids from the upper phase were re-extracted with 400 µl CHCl 3 :MeOH (2:1, vol/vol), vortexed (10 s) and 100 µl of H 2 O was added to promote phase separation, followed by vortexing (10 s), centrifugation and collection of the lower phase (380 µl), which was combined with the first extract and dried as above

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10.3389/fphar.2018.01371 203 SunC.LiuP.PeiL.ZhaoM.HuangY