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In agreement with this conclusion, CTIF and DCTN1 co-immunopurified with FLAG-eEF1A1 in a way that was unaffected by treatment with MG132 (Supplementary Fig
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and preferably a fourth container containing a reducing agent, selected from the group consisting of phosphine-based reducing agents such as TCEP or tributylphosphine, and thiol-based reducing agents such as DTT, DTE or β-mercaptoethanol
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