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First, 100 uL samples and standard controls were added to each well in the antibody-coated plate and incubated for 2 hours at 37 °C in the incubator

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268 For example, high levels of Cu have been found in senile plaques and serum in patients with AD, 269,270 and high concentrations of Cu have also been measured in Aβ plaques in the dentate gyrus subregion of the hippocampus in a mouse model of AD