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Specific research directions include: Energy metabolism of adipose tissue — studies on the impact of NNMT inhibition on adipocyte energy expenditure and lipid metabolism in obesity models Nicotinamide/NAD⁺ axis — measurements of how inhibition of nicotinamide methylation translates into the availability of substrate for NAD regeneration via the salvage pathway Management of methyl groups — analysis of the impact of NNMT inhibition on the SAM/SAH balance and, secondarily, on other methylation reactions Regeneration and muscle mass — research on the role of NNMT in skeletal muscle satellite cells Structure-activity relationships (SAR) — characterization of 5-Amino-1MQ as a representative of small-molecule NNMT inhibitors in comparative analyzes When designing comparative experiments on the metabolism of adipose tissue, 5-Amino-1MQ is sometimes combined with compounds with a different molecular target – for example, with a lipolytic fragment of growth hormone AOD-9604 or an oral incretin agonist GLP-1 MAX