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Full article: Metformin ameliorates acetaminophen-induced sub-acute toxicity via antioxidant property

Key metrics include mtDNA copy number (reflecting depletion), mtDNA deletion frequency measured through long-fragment PCR or digital PCR, and levels of oxidized mtDNA bases like 8-oxo-dG (111, 120)

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8.4 Final perspective The convergence of evidence from molecular studies, epidemiological investigations, and intervention trials establishes environmental endocrine disruptors as significant threats to male reproductive health with implications extending far beyond individual exposure events