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Moreover, given that drug exposure is expected to be highest in the olfactory bulbs compared to other brain regions, IN chelator therapy may be particularly beneficial for disorders involving early olfactory bulb pathology or localized iron dysregulation near the nasal region ( 5.1 Mechanism of intranasal delivery Since William Frey II proposed intranasal administration in 1991 (Frey, 1991), this technique has emerged as a novel, non-invasive delivery system, bypassing the BBB and the systemic first-pass effect, allowing rapid onset of action

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The strategy adopted has used the Strep -tagged GSTs as protein ‘hooks,’ which when either expressed in planta transiently ( Nicotiana benthamiana ), or stably (Arabidopsis) bind endogenous ligands in the host which can then be recovered following affinity capture of the fusion protein (Figure 1)