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Peptide Sciences IGF1 LR3 действует как ауто- и паракринный гормон, при этом изменение его содержания в тканях совершается без трансформации его концентрации в крови, а это изменение модулируют связывающие белки

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Total proteins were incubated in 30 µL of kinase buffer (20 mM Tris–HCl, pH 8.0, 1 mM DTT, 5 mM MgCl 2 , and 10 μM ATP) at 30 °C for 30 min, His-tagged PKS5 were removed by a 5-min centrifugation at 500 × g at 4 °C