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Two days later, GLP-1, dulaglutide, DCA, forskolin, L-glutamine, sodium butyrate, and 007-AM (8-pCPT-2-O-Me-cAMP-AM), as well as glucose in Hank’s Balanced Salt Solution (HBSS) containing 0.01% (v/v) bovine serum albumin (BSA), 5-mM HEPES, and 0.5-mM 3-isobutyl-1-methylxanthine (IBMX), were applied to the cells at a volume of 50 µL per well and incubated for 60 min

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This imbalance occurs because the body senses too much thyroid hormone in the bloodstream, signaling the pituitary gland to reduce TSH release in an attempt to restore hormonal balance