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GPX4, also known as phospholipid hydroperoxide GSH-Px, is the fourth member of the selenium-containing GSH-Px family, with a molecular weight of approximately 20–21 kDa and composed of about 197 amino acids (Pei et al., 2023)

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Indeed, the mixture of E2E1-I53-50A with I53-50B.4PT1 eluted substantially earlier from the Superose 6 column than E2E1-I53-50A trimer alone indicating successful nanoparticle formation (~9.0 mL versus ~14.5 mL for E2E1-I53-50A) (Fig