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Trigger Point Injection for Myofascial Pain Syndrome in the Low Back: A Randomized Controlled Trial ( Trial number: NCT04704297 Overview: This study is testing whether adding trigger point injections (TPI) with a local anesthetic called Bupivacaine to standard therapy helps reduce pain and improve function in adults with myofascial pain syndrome (a type of muscle pain) in the low back, compared to standard therapy alone or with TPI using saline

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Each sample (8 μl) was injected onto an Agilent Poroshell 120 EC-C18 (100 × 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature

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