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Further investigation into the testicular microenvironment using a co-culture system of senescent TM3 Leydig cells and GC-1 spg spermatogonia revealed that H2O2-induced senescent TM3 cells secreted increased levels of SASP factors, particularly IL-6 and TGF-β
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Because a large number of comparisons were performed on the relative abundance data, main effect results from these analyses also underwent a Benjamini–Hochberg false discovery rate (FDR) correction for multiple comparisons, and instances where a result was significant after this correction have been duly noted
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